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Artificial intelligence (AI) is a rapidly developing technological development that helps individuals in many areas. However, this progress is met with different reactions from individuals. The paper aims to investigate the associations among the concepts of spiritual intelligence, intolerance of uncertainty and AI anxiety. Furthermore, mediating role of intolerance of uncertainty on the relationship between spiritual intelligence and AI anxiety is another aim to examine in this study. In order to measure the mentioned variables, data was collected from 382 white-collar employees using survey method. We conducted explanatory and confirmatory factor analyses to control validity, and Crombach Alpha values to control reliability of the scales. Findings represented that each scale was valid and reliable. After that measurement model and structural equation modelling was conducted for model testing. The results represented the significant relationship between spiritual intelligence and intolerance of uncertainty, between uncertainty tolerance and AI anxiety (also its dimensions). In addition, findings showed that intolerance of uncertainty fully mediates the relationship between spiritual intelligence and learning of AI anxiety, also between job replacement of AI anxiety. Furthermore, results presented that intolerance of uncertainty partially mediates the relationship between spiritual intelligence and sociotechnical blindness and configuration of AI anxiety.