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Microenterprises are crucial in sustaining the economy, especially in developing economies like Nigeria. Many of these microenterprises do not take cognizance of necessary determinants like organizational culture and its factors to ensure proper running of their day-to-day activities. The study aims to identify some demographic statistics of employers in technological microenterprises, investigate the factors influencing organizational culture and assess organizational culture's influence on business success among employers in selected technological microenterprises in southwest Nigeria. The study was carried out in three southwestern states in Nigeria with 213 business owners (employers) as respondents. Seventeen factors were explored, but eight were extracted to be considered in measuring the employers' organizational culture: climate, customer demand, technological changes, observed behaviour, norms, consistency, adaptability, and sense of mission. Linear regression analysis was also used to measure organizational culture's impact on technological microenterprises' business performance with SPSS as a statistical tool.   The study confirms a positive and significant relationship between organizational culture and business performance at 5% significance. This study concludes that the eight variables extracted are important factors to consider when measuring the organizational culture of employers in technological microenterprises in southwest Nigeria for better business production.